Molecular insight into the expression level of an immunosuppression gene, VTCN1, and its regulatory factors in breast cancer patients and non-cancerous ...
This study investigated VTCN1 and the lncRNA NORAD as potential breast cancer biomarkers in tumor tissues and blood samples from individuals with high IgE levels, using real-time PCR, bioinformatics analyses, protein interactions, and pathway enrichment to explore their diagnostic relevance.
Adel Rezvani Sichani, Pouya Dadkhah, Tara Tabandeh, Nafiseh Kaviani Dehkordi, Mohammad Rezaei, Samira Rahimirad, Mansoureh Azadeh · October 21, 2026
IgE levels increase in some cancer types due to treatment methods also autoimmune diseases can emerge and lead to worsening of the cancer. Finding a factor based on the reduction in IgE levels found in cancer can act as a determinant in providing the early diagnosis of the disease in individuals with high IgE levels in their blood. Accordingly, in the present study, the effort was made to select a gene associated with breast cancer and high IgE levels through available databases to evaluate the gene expression and the associated lncRNA and polymorphism at the binding site of a miRNA with a gene.
In this study, the expression of the VTCN1 gene and the associated lncRNA called NORAD (Non-Coding RNA Activated by DNA Damage), selected through bioinformatics methods and relevant databases were assessed in breast cancer tissue samples along with the blood samples of individuals with high IgE levels, employing the real-time PCR method. Lastly, NORAD’s protein interactions and the relevant signaling pathways were further investigated via bioinformatics tools.