lncRNAs ITFG2-AS1 and MMP25-AS1 have significant high-expression in gastric cancer patients as the potential diagnostic biomarkers: Integrated bioinformatics...
This study integrated bioinformatics and experimental analyses to investigate two novel long non-coding RNAs in gastric cancer, assessing their expression, regulatory interactions, and associated pathways to explore their potential as diagnostic and prognostic biomarkers.
Fatemeh Mohebi, Zahra Zamanzadeh, Morteza Abkar, Mansoureh Azadeh · October 25, 2026
Background
The second most frequent cause of cancer-related death is gastric cancer (GC). Recent studies have reported that non-coding RNAs seem to play a crucial role in many tumors. Due to their extraordinary stability, long non-coding RNAs (lncRNAs) have the potential to be employed as biomarkers to identify the presence and prognosis of GC. In this study, we performed an integrated bioinformatics and experimental approach to evaluate the expression and interaction of two novel lncRNAs in the GC samples.
Methods
Bioinformatics analyses was performed to demonstrate the lncRNA expression profile of gastric cancer patients. Microarray data analysis was executed by R Studio, using affy and limma packages. Validation of gene expression analysis was carried out by the ENCORI online database. Pathway enrichment analysis was conducted using enrichr and Reactome. Protein interaction analysis was …