Evaluation of the cytotoxic effect of GaFe2O4@Ag magnetic nanocomposite biosynthesized by Spirulina platensis on breast cancer cell line and evaluation of ...
GaFe₂O₄@Ag nanocomposite biosynthesized using Spirulina platensis was evaluated for cytotoxicity and apoptosis induction in breast cancer MCF-7 cells. Cell viability, apoptosis, caspase-3 activity, and expression of BAX, Bcl-2, CASP9, and MMP1 were assessed.
Fatemeh Mehraban, Fariba Heidari Esfahani, Zahra Mosayebi Dorcheh, Erfaneh Heidari Esfahani, Mansoureh Azadeh, Ali Salehzadeh · October 21, 2026
Treating breast cancer is hardly achieved and finding efficient anticancer nanocompounds has gained great attention. In this work, the cytotoxic effect of GaFe2O4@Ag nanocomposite biosynthesized by Spirulina platensis on breast cancer cell line, and expression of the BAX, Bcl-2, CASP9 and MMP1 (Matrix Metallopeptidase 1) genes were evaluated. Physicochemical features of the nanocomposite were determined using the FT-IR, XRD, SEM, TEM, EDX-mapping, VSM, Zeta potential, and DLS analyses. The cytotoxic effect of the nanocomposite for MCF-7 and HEK-293 cells was evaluated by the MTT assay. Flow cytometry analysis, Caspase-3 activation assay, and Hoechst staining were performed to evaluate the apoptosis induction potential of the nanocomposite. Further, the relative expression of the Bcl-2, BAX, and CASP9, and MMP1 genes was determined by quantitative PCR assay. The prepared …