Altered expression level of ACSM5 in breast cancer: an integrative analysis of tissue biomarkers with diagnostic potential
This study investigated fatty acid activation in breast cancer by analyzing ACS enzyme expression in TCGA and tissue samples. Differential expression and bioinformatics analyses aimed to identify potential biomarkers and regulatory lncRNAs associated with altered fatty acid metabolism.
Behnaz Yazdani, Mahnoosh Jazini, Negin Jabbari, Marjan Karami, Samira Rahimirad, Mansoureh Azadeh, Mohammad Mahdevar, Kamran Ghaedi · October 20, 2026
gene expression levels of enzymes involved in fatty acid synthesis and oxidation pathways in breast cancer have been shown to associate with progression of breast cancer. However, little is known about the significance of alternations in fatty acid activation, as a key step in their metabolism. We aimed to recognize suitable biomarkers indicating the altered activation of fatty acids and examine their differential expression in breast cancer and adjacent normal breast tissue samples.
Material and methods
Transcript levels of ACS enzymes were analyzed in TCGA data (RNAseq), breast cancer and normal breast samples by differential gene expression (DGE). Gene set enrichment analysis (GSEA) was performed to highlight the most enriched genes involved in the activation of fatty acids. Bioinformatic analysis was performed for the selection of LncRNAs. Gene expression analyses were …