LINC02544 modulates the RAB/RAS signaling pathway by potential regulation of DENND2A expression level in the breast cancer patients: integrated ...
This study investigated DENND2A and LINC02544 expression in Iranian breast cancer tissues using bioinformatics and RT-qPCR. DENND2A expression was reduced in tumors, while HRM analysis assessed the rs6852 polymorphism, exploring their potential as breast cancer biomarkers.
Maria Nezamnia, Sayedeh Zahra Shirdeli, Fatemeh Sobhani, Mina Moein, Samira Rahimirad, Mansoureh Azadeh, Sara Azhdari · October 21, 2026
Background: One of the most common female malignancies is breast cancer (BC) and is recognized as a second death factor for women population among other cancer-related diseases. Dysregulation of RNA expression levels can lead human status to some different pathological statuses, including breast cancer. In this study, we have investigated the expression level of DENND2A and lncRNA LINC02544 in the BC tissue samples among Iranian population. Also, the clinicopathological and biomarker analysis of these RNAs were investigated.
Method: Microarray analysis was performed on GSE61304 in order to nd the differentially expressed genes in the BC samples compared to controls. Multiple online and o ine software, including R programming language and several relative statistical and visualization packages, demonstrated the differential expression level of genes, relative lncRNAs, and single nucleotide polymorphisms. The Realtime PCR results have demonstrated that the expression level of DENND2A has been reduced in the breast cancer samples compared with controls. In order to investigate the genotype frequency of rs6852 region in the DENND2A gene, high-resolution melt (HRM) method has been used.