LNC01089-LINC00963/miR-1244-5p/IGF1 ceRNA axis might regulate FOXO signaling pathway in breast cancer patients: A biomarker discovery investigation
This study uses a systems biology approach to investigate mRNA and lncRNA expression patterns in breast cancer, aiming to identify novel molecular biomarkers for improved diagnosis and prognosis of the disease.
Mohammad Rezaei, Ramin Masoudi Marghmaleki, Farzaneh Sanati Boroujeni, Abbas Shahriari, Shadi Omidghaemi, Mansoureh Azadeh, Ashkan Golshani · October 21, 2026
Background
Breast carcinoma (BC) ranks as one of the most prevalent illnesses among women, and a variety of factors, including inherited and environmental factors, can impact its start and progression. In this study, we tried to analyze the expression patterns of mRNAs and long noncoding RNAs and find novel biomarkers for the diagnosis and prognosis of BC during a systems biology approach.
Materials and Methods
Microarray analysis was performed to find novel potential BC biomarkers. Using miRWalk, lncRRIsearch, STRING, and Cytoscape, noncoding and protein interaction analysis was utilized and visualized. Pathway enrichment and gene ontology analyses were performed to find accurate biological mechanisms of selected RNAs. qRT-PCR was established on 50 tumor samples compared to 50 control samples for validation of bioinformatics analyses and understanding of the diagnosis capability of …