Evaluation of BHMT2 gene expression and its expression factor regulating LNC00922 LNCRNA with rs10944 genotype determination in patients with breast cancer
This study investigates BHMT2 and LINC00922 expression in breast cancer using bioinformatics, interaction and pathway enrichment analyses, and evaluates BHMT2 rs10499, a SNP potentially affecting miR-542-3p binding affinity.
Seyed Ali Dibaj Zavareh, Zahra Akhlaghi, Sarina Samiei Esfahani, Niloofar Mohammadi, Samira Rahimirad, Mansoureh Azadeh · October 21, 2026
Background: It is predicted that dysregulation of BHMT2 expression level could have a remarkable effect on Breast cancer status. The absence of methionine and Hyperhomocysteinemia have been linked to a decline in BHMT2 expression. This mRNA expression and its correlated long non-coding RNA (lncRNA)(LINC00922) have been investigated in our experiment based on the bioinformatics approach. Also, the genotype frequency analysis of BHMT2 in SNP rs10499 has been demonstrated. The mentioned SNP is correlated with the differences in the binding affinity of miR542-3p.
Material and method: we performed a microarray analysis to find the differentially expressed genes (DEGs). GeneMANIA and miRWalk online databases were used for mRNA-mRNA and mRNA-miRNA interaction analyses. Clusterprofiler package was used for functional and pathway enrichment analyses. dbSNP database was applied for finding the relevant Single nucleotide polymorphism